Ischemic stroke is considered as a leading cause of mortality and long-term disability worldwide. Several potential biomarkers were previously studied in relation to stroke, among them; serum uric acid (SUA) that have attracted a significant attention owing to its dual role seen as an antioxidant and a pro-oxidant. The current study was designed as a cross-sectional observational study that was conducted at Baquba Teaching Hospital, Diyala, Iraq, over a period from November 2024 to April 2025. The study was aimed to investigate the association between SUA levels and the incidence of ischemic stroke, severity and the disease outcomes. The current study included 56 Iraqi patients were diagnosed with stroke at Baquba Teaching Hospital, Diyala, Iraq and 44 healthy subjects recruited as controls. Blood samples were collected and SUA levels were measured using enzymatic methods. Statistical analysis revealed no significant difference in uric acid levels between healthy and abnormal subjects. Furthermore; no significant correlations were recognized between each of; SUA and glucose, blood pressure, age, or body weight. However, the study revealed a moderate positive correlation between SUA and serum urea levels. The obtained findings proposed that SUA may not serve as a direct risk factor for incidence of ischemic stroke, despite its role still complex and may vary depending on accompanying comorbid conditions. Further longitudinal with larger sample size studies are required to elucidate its predictive and therapeutic significance.