Background: Cardiovascular diseases represent a major worldwide health issue, and avocados, abundant in monounsaturated fats and bioactive substances, may enhance heart health by affecting lipid profiles and additional risk determinants. Objective: Determine the effectiveness of different concentrations of avocado in improving the health of hyperlipidemic rats. Materials and methods: Twenty-four male Sprague-Dawley albino rats, aged 10 weeks, weighing 150±10g, were split into 4 groups. The experiment was carried out in two periods. In the first period (three weeks), the first group was fed as a control group and was fed a control diet, while the other groups were fed the hyperlipidemic diets. In the second period (6 weeks), one of the hyperlipidemic rats fed on a hyperlipidemic diet (10% animal fat and 1%. cholesterol). While the other groups were fed on a hyperlipidemic diet supplemented with different levels of avocado (Persea americana), while one group was given a regular diet to act as a control positive. The experiment concluded with a blood sample and biochemical examination of the excised organs. Results: Serum free fatty acids, Serum total cholesterol, Serum phospholipids and Serum triglycerides there were significant differences (p < 0.01) between untreated group G2 and the other groups under investigation. Serum urea and Serum creatinine decreased significantly (p < 0.01) of rats fed on avocado (5 and 10%) when compared with untreated groups (G2). Serum Ca, Serum p, Serum Mg, and Serum Fe in untreated groups (G2) were decreased significantly (p < 0.05) when compared with other groups supplemented with different levels of avocado. Conclusion: Avocado's bioactive components, like monounsaturated fatty acids, flavonoids, and phenolic compounds, contribute to its health benefits. Additionally, the antioxidants and minerals in avocado support liver and kidney function, promoting overall metabolic health.