Background: Pseudomonas aeruginosa is an opportunistic bacterium that causes infections via a variety of mechanisms, including as biofilm development and medication resistance. Aim: Examining the biofilm patterns and antibiotic sensitivity of P. aeruginosa isolated from burn and wound patients was the aim of the current study. Methods: At Samarra Hospital in Samarra, Iraq, 163 clinical specimens from burn and wound patients have been collected. The isolates were identified using both traditional techniques and the VITEK 2 technology. The microtiter plate (MTP) method was used to detect biofilm development, and the isolates underwent the antibiotic susceptibility test (AST).Results: Forty P. aeruginosa isolates were obtained from 163 clinical specimens, of which eighteen (45%) were from burns and twenty-two (55%) were from wound swabs. The results revealed high rates of resistance to Colistin (98%), Piperacillin-tazobactam (90%), Ceftazidime (88%), and Meropenem (80%), as well as moderate levels of resistance to Tigecycline (73%) and Aztreonam (63%). Resistance rates to gentamicin (8%), amikacin (10%), and ciprofloxacin (34%) were low. The biofilm test revealed that 57.5% of the isolates formed strong biofilms, 20% formed intermediate biofilms, and 7.70% formed weak biofilms. Conclusion: Antibiotic resistance has been observed to positively correlate with the highly proportional potential for biofilm formation displayed by clinical isolates of P. aeruginosa.