Background: Inguinal hernia repair triggers an inflammatory and neuroendocrine stress response that varies with the surgical technique. Interleukin-6 (IL-6) and Adrenocorticotropic hormone (ACTH) serve as sensitive markers for assessing the extent of physiological stress. Comparative biochemical responses following laparoscopic TAPP and open Lichtenstein repair remain inadequately studied, particularly at the 48-hour postoperative interval. Aim: To evaluate and compare the rise in IL-6 and ACTH levels following laparoscopic TAPP versus Lichtenstein repair for inguinal hernia. Methods: A prospective analytical study was conducted on 26 patients with inguinal hernia, divided equally between Lichtenstein repair (n=13) and TAPP repair (n=13). Preoperative and 48-hour postoperative blood samples were analyzed for IL-6 using ECLIA and ACTH using ELISA. Demographic data, including age and gender distribution, were recorded. Statistical analysis was performed using appropriate parametric and non-parametric tests to compare changes between groups. Results: Both groups demonstrated postoperative elevation of IL-6 and ACTH. The mean IL-6 rise was slightly higher in the TAPP group (14.75 pg/ml) than in the Lichtenstein group (11.64 pg/ml). ACTH showed a markedly greater increase in the TAPP group (65.22 pg/ml) compared with Lichtenstein repair (14.06 pg/ml). However, these differences were not statistically significant (ΔIL-6: p>0.5; ΔACTH: p>0.3). Multivariate regression indicated that younger patients undergoing TAPP exhibited a stronger surge in both markers. Conclusion: Both Lichtenstein and TAPP inguinal hernia repairs elicit inflammatory and endocrine stress responses, with TAPP demonstrating a higher numerical rise in IL-6 and a substantially greater ACTH surge at 48 hours. Although these differences were not statistically significant in this small cohort, the trends suggest that pneumoperitoneum and peritoneal handling in TAPP may contribute to enhanced physiological stress. Larger, multi-time-point studies are needed to determine the clinical relevance of these biochemical changes.