Background: In vitro experiments have demonstrated that cell alignment by underlying topographical cues influences crucial biological processes like differentiation and functional maturation.Optical media, such as CD-R, DVD-R, and optical grating, provide good surfaces for many cell types to adhere to and develop.The physical dimensions of the grooves in these optical media allowed for confluent cell culture with maximum cell cell contact. AIM: The aim of this study is to assess the suitability of DVD discs for cell growth and differentiation. MATERIALS AND METHODS: For culturing osteoblast cells, the DVDs were processed with 70% ethanol for one hour and then autoclaved at 121 C for 15 minutes. Micrographs of data-written and data-unwritten DVD images were scanned. Measurements of the water contact angle on DVD surfaces that had been exposed to oxygen plasma and air at various times were measured. RESULTS AND DISCUSSION: It was observed that in light microscopy, Tissue Culture Plates (TCP) have a random orientation, whereas DVD discs have an anisotropically oriented architecture. In fluorescent microscopy, in TCP, the cells have a random orientation, whereas DVD discs have an isotropically aligned architecture.Commercial optical media are designed for spectroscopy and data storage, thus it is crucial to prepare them so that they are compatible with cell culture. CONCLUSION: This study concludes that DVDs can be used for these investigations and applications as scalable cell alignment substrates with micro- and nano-topography and is suitable for cell growth and differentiation.