Research Approach: Quantitative research approach was being used for the current study. The research was being conducted among staff nurses working in selected community health setting. Research Design: A pre-test post-test control group design was adopted to accomplish the main purpose of the study i.e. to assess the effectiveness of assertiveness training programme on assertive behavior. Sample: Total 220 samples were selected (Experimental group:110 & Control group:110). Sampling Techniques: It was a three steps process. Mixed Sampling techniques (Selection of District, Selection of Talukas, Selection of PHC and SC). Research Tool: A Self Structured Assertiveness Inventory Scale was to used collect data. Self-Structured Assertiveness Inventory Scale was used in this study to assess the assertiveness skills and nurse’s impression of one’s own assertiveness and frankness. Items are answered on a four-point rating scale from ‘Never’ to ‘Entirely’. It contains 25 items. Results: The training regimen was structured as a two-day program, with sessions spanning four hours each day. Over the course of the training, participants engaged in eight distinct sessions covering topics such as understanding assertiveness, cultivating assertive thinking, learning various assertive techniques, practicing refusal skills for unreasonable requests, managing criticism, and stress reduction techniques. In Experimental group 87.3% of participants showed assertive behavior. Fisher Exact Test value: 217.54, p-value shows 0.000 which is less than 0.05 which indicates a highly significant difference.