Urinary tract infections (UTIs) form one of the highly prevailing infections of bacteria all over the world and southern Iraq including the Thi Qar Province exhibits a very high number of the latter. Though UTIs are prevalent, few region facts are available on host immune reaction, and particularly on the element of innate immunity. In this research, we were interested in examining the correlation between bacterial uropathogens and the parametres of the innate immune system, i.e. interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-a), neutrophil counts, and Toll-like receptor 4 (TLR4) gene expression, among UTI patients in Thi Qar.The research group took 240 samples of urine from patients who showed symptoms and attended medical facilities in Nasiriyah. Standard culturing and biochemical methods were used in the identification of bacterial pathogens. Cytokine levels in serum were determined by ELISA, whereas the TLR4 expression was determined by real-time PCR. Complete blood count analysis was used to get neutrophil counts. The most commonly identified pathogen was Escherichia coli (61.9%), thereafter Klebsiella spp., Proteus mirabilis and Pseudomonas aeruginosa. Elevated IL-6 and TNF-a levels were recorded in 72.1 and 64.6 of the patients respectively. Expression of TLR4 was found to be significantly high in patients infected with P. aeruginosa showing stronger innate activation. In 76.1 percent of cases, neutrophil elevation was noted. Statistical analysis indicated the significant correlation of TLR4 expression with IL-6 (r = 0.66) and the correlation of IL-6 with TNF-a (r = 0.72) as well, which confirms the coordinated innate immune response.These data shows the crucial importance of the role of innate immunity in the pathogenesis of UTI in Thi Qar and deserves consideration because the immune markers could underlie the better correct diagnosis and effective treatment.